|
KMID : 0613820110210091226
|
|
Journal of Life Science
2011 Volume.21 No. 9 p.1226 ~ p.1233
|
|
|
The STAR RNA Binding Proteins SAM68, SLM-1 and SLM-2 Interact with Kinesin-I
|
|
Seog Dae-Hyun
|
|
|
Abstract
|
|
|
|
In neurons, kinesin is the molecular motor that transport cargos along microtubules. KIF5s (alias kinesin-I), are heterotetrameric motor conveying cargos, but the mechanism as to how they recognize and bind to a specific cargos has not yet been completely elucidated. To identify the interaction proteins for KIF5C, yeast two-hybrid screening was performed, and specific interaction with the Sam68-Iike mammalian protein 2 (SLM-2), a member of the Signal Transducers and Activators of RNA (STAR) family of RNA processing proteins, was found. SLM-2 bound to the carboxyl (C)-terminal region of KIF5C and to other KIF5 members. The C-terminal domain of Sam68, SLM-1, SLM-2 was essential for interaction with KIF5C in the yeast two-hybrid assay. In addition, glutathione S-transferase (GST) pull-downs showed that SAM68, SLM-1, and SLM-2 specifically interacted to Kinesin-I complex. An antibody to SAM68 specifically co-immunoprecipitated SAM68 associated with KIF5s and coprecipitated with a specific set of mRNA. These results suggest that Kinesin-I motor protein transports RNA-associated protein complex in cells.
|
|
|
KEYWORD
|
|
|
Kinesin-I, molecular motors, RNA, RNA binding protein
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|